Column Chromatography

Line Chromatography of Plant Pigments

Jaybee Balilea, Sharmaine Baysic, Maria Anjelette Patricia Belen 3Bio-7, Department of Biological Sciences, College or university of Imagen Tomas, Manila, Philippines


Column Chromatography is a form of solid-liquid adsorption chromatography and depends on the necessary principles along with in slim layer chromatography. It was utilized in this try things out in distancing and studying the different pieces of Capsicum frutescens (siling labuyo) with the use of solvents such as Hexane (C6H14), Dichloromethane (CH2Cl2), Hexane/Dichloromethane (1: 1), and Dichloromethane/Methanol (MeOH) (1: 1). The components found in the experiment had been carotenoids (carotenes and lutein), xanthophylls (capsanthin) and anthocyanins.


[1]Column Chromatography is a solid-liquid adsorption chromatography where a multicomponent mixture is normally dissolved in a small amount of your appropriate solvent and placed on the top of your packed line containing a finely divided, active sound adsorbent that serves as the stationary phase. An eluant or eluting solvent that serves as the mobile period is passed down the column. The individual pieces of a mixture which were adsorbed around the stationary stage at the top of the column begin to move down with the eluting solvent. These kinds of components travelling at different rates according to their comparable affinities to get the packing material. The greater weakly the compound is usually adsorbed, the faster it will be eluted from the column than the usual more strongly adsorbed substance. Components are placed in individual test pipes or storage units as they leave from the bottom end of the steering column in bands. Column Chromatography can be used in both analytical and preparative applications. It is used to individual and purity substantial quantities of those parts for following analysis.

Column Chromatography was applied in distancing the component components of Capsicum frutescens. Capsicum frutescens is composed of different constituents such as alkaloids: capsaicin, carotenoids and other tones, Vitamin A, Vitamin C and other bio-active compounds. This can be a rich supply of iron and calcium. It is just a good anti-fatigue remedy and it helps to improve human's disease fighting capability.

This try things out aims to distinct and examine the different pieces of Capsicum frutescens with the use of Steering column Chromatography.

Results and Discussion

Inside the experiment, Chili peppers frutescens was chopped into smaller parts so that it will be easy to remove its elements. These sliced siling labuyo was put in a mortar and pestle with yellow sand on it. Yellow sand was used to homogenize the extract. In that case 3ml dichloromethane (DCM) was added, the specified components of siling labuyo had been soluble in DCM. Following your addition of DCM, the extract was decanted using funnel into the evaporating dish. After that, a great anhydrous salt was added in the grow extract to dry impurities and to promote spontaneous evaporation. Following few minutes, Hexane was added in the producing dry herb extract to liquefy and at the same time to dissolve the sample and the wanted components. Then the glass dropper with cotton inside was used as a steering column. Cotton was inserted to do something as a shock absorber. Then a quite dense silica gel was placed in the dropper to serve as the polar stationary phase. Before starting the process and also the column elution, the column with silica gel and cotton was rinsed with hexane that produces a slurry texture. After rinsing, the red plant extract was added drop by drop in the column. Then simply hexane was placed again at the top of the column to act as the first portable phase solvent. Different solvent was added continuously till a color band descends. Hexane can be described as nonpolar aspect that treats the non-polar component inside the sample. This non-polar portable phase quickly passed through the stationary phase and eluted faster over the column. Mainly because it elutes throughout the column, colour it carried along was yellow. This kind of yellow...

References: [1] Gilbert, J. & Martin S i9000., " Organic Chemistry Lab Experiments”. sixth edition, Cengage

Learning, USA, В©2011, pp. 171-185

[2]Retrieved on July 40, 2011 by World Wide Web: http://www.chem.ubc.ca/courseware/121/tutorials/exp3A/columnchrom/

[3]Retrieved upon July 30, 2011 from World Wide Web: http://www.mctony.com/columnChromatography./html



Sonicwall Article

29.08.2019 SonicWALL TZ 215 Series FIR Elizabeth WALL The highest-performing, best UTM firewall for tiny offices and High performance security engine Bundled intrusion prevention Advanced…..